WHAT IS ARTIFICIAL OOCYTE ACTIVATION(AOA)?
- When a sperm meets an egg, it triggers something called ‘egg activation’ which begins the process of fertilisation and embryo development. This activation process is caused by sperm factors which lead to an increase of calcium in the oocyte.
- If the sperm are lacking these factors or the oocytes don’t respond to the factors properly, then the activation process may not occur and fertilisation can fail.
- The ICSI procedure improves fertilization rates in cases of male factor infertility, fertilization failure still occurs in 1-5% in ICSI cycles. The main cause of failed fertilization is failure to complete oocyte activation.
- Artificial oocyte activation (AOA) is an effective method to avoid total fertilization failure in IVF/ICSI cycles.
WHAT IS ARTIFICIAL OOCYTE ACTIVATION USING CALCIUM IONOPHORE?
- At present, several methods are available to activate the oocytes through physical and chemical stimulation, including electromechanical stimulation, calcium activation [calcium ionophore (A23187) or ionomycin], strontium chloride activation, phospholipase C zeta (PLCζ) activation
- The use of calcium ionophore (A23187) is one of the most efficient oocyte activation methods widely used in clinical practice which aims to help to activate the egg and improve fertilisation.
- Calcium ionophore( A23187), which can mimic the natural pattern of calcium rise resulting in effectively increasing intracellular calcium levels which can activate oocytes, initiate fertilization and help obtain normal embryos.